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16S rRNA gene-based analysis of microbial community by whole-genome amplification and minigel-single-strand conformation polymorphism technique

Paper ID Volume ID Publish Year Pages File Format Full-Text
22525 43300 2006 3 PDF Available
Title
16S rRNA gene-based analysis of microbial community by whole-genome amplification and minigel-single-strand conformation polymorphism technique
Abstract

We have developed an analytical technique for the 16S rRNA gene that comprises whole-genome amplification and the polymerase chain reaction (PCR)-minigel-single-strand conformation polymorphism technique (WGA-SSCP). Under optimal conditions, SSCP bands could be detected when genomic DNA from bacteria of interest comprised 0.5% or more of the specimen. This method will be effective for the identification of nonculturable bacteria in a microbial community.

Keywords
whole-genome amplification (WGA); polymerase chain reaction (PCR); single-strand conformation polymorphism (SSCP); 16S rRNA gene; nonculturable bacteria
First Page Preview
16S rRNA gene-based analysis of microbial community by whole-genome amplification and minigel-single-strand conformation polymorphism technique
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 102, Issue 5, November 2006, Pages 482–484
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering