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The corn smut-made cholera oral vaccine is thermostable and induces long-lasting immunity in mouse

Paper ID Volume ID Publish Year Pages File Format Full-Text
22528 43364 2016 6 PDF Available
Title
The corn smut-made cholera oral vaccine is thermostable and induces long-lasting immunity in mouse
Abstract

•Stability and immunogenicity of a corn smut CTB-based cholera vaccine are reported.•The vaccine induced toxin neutralizing IgA responses in mice at 15 μg doses.•The vaccine is highly thermo stable and induced long lasting immunity.

The use of corn smut for the production of recombinant vaccines has been recently implemented by our group. In this study, the stability and immunogenic properties of the corn smut-based cholera vaccine, based on the cholera toxin B subunit (CTB), were determined in mouse. The immunogenic potential of distinct corn smut CTB doses ranging from 1 to 30 μg were assessed, with maximum humoral responses at both the systemic (IgG) and intestinal (IgA) levels at a dose of 15 μg. The humoral response last for up to 70 days after the third boost. Mice were fully protected against a challenge with cholera toxin after receiving three 15 μg-doses. Remarkably, the corn smut-made vaccine retained its immunogenic activity after storage at room temperature for a period of 1 year and no reduction on CTB was observed following exposure at 50 °C for 2 h. These data support the use of the corn smut-made CTB vaccine as a highly stable and effective immunogen and justify its evaluation in target animal models, such as piglet and sheep, as well as clinical evaluations in humans.

Keywords
CTB, cholera toxin B subunit; CT, cholera toxin; FA, fluid accumulationCholera; Low-cost vaccine; Oral immunization; Cold chain; Neutralizing antibodies; Cholera toxin
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The corn smut-made cholera oral vaccine is thermostable and induces long-lasting immunity in mouse
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 234, 20 September 2016, Pages 1–6
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us