fulltext.study @t Gmail

Complete genome analysis of Clostridium bornimense strain M2/40T: A new acidogenic Clostridium species isolated from a mesophilic two-phase laboratory-scale biogas reactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
22534 43366 2016 12 PDF Available
Title
Complete genome analysis of Clostridium bornimense strain M2/40T: A new acidogenic Clostridium species isolated from a mesophilic two-phase laboratory-scale biogas reactor
Abstract

•Presentation of the genome sequence for the new type strain Clostridium bornimense M2/40T.•Strain C. bornimense M2/40T harbors a chromid encoding active glycoside hydrolase enzymes.•Reconstruction of fermentation pathways with importance for biofuel production from the genome sequence.

Taxonomic and functional profiling based on metagenome analyses frequently revealed that members of the class Clostridia dominate biogas reactor communities and perform different essential metabolic pathways in the biogas fermentation process. Clostridium bornimense strain M2/40T was recently isolated from a mesophilic two-phase lab-scale biogas reactor continuously fed with maize silage and wheat straw. The genome of the strain was completely sequenced and manually annotated to reconstruct its metabolic potential regarding carbohydrate active enzyme production and fermentation of organic compounds for consolidated biofuel production from biomass. The C. bornimense M2/40T genome consists of a chromosome (2,917,864 bp in size) containing 2613 protein coding sequences, and a 699,161 bp chromid (secondary replicon) harboring 680 coding sequences. Both replicons feature very similar GC-contents of approximately 29%. The complex genome comprises three prophage regions, two CRISPR-cas systems and a putative cellulosomal gene cluster that is located on the second replicon (chromid) of the strain. The overexpressed glycosyl hydrolases (GH) CelK (GH9) and CelA (GH48) encoded in the cellulosomal gene cluster were shown to be active on the substrates xylan and xyloglucan whereas XghA (GH74) is highly active on xyloglucan. Reconstruction of fermentation pathways from genome sequence data revealed that strain M2/40T encodes all enzymes for hydrogen, acetate, formate, lactate, butyrate, and ethanol production, leading to the classification of the isolate as acidogenic bacterium. Phylogenetic analyses uncovered that the closest characterized relative of C. bornimense is C. cellulovorans. Comparative analyses of the C. bornimense and C. cellulovorans genomes revealed considerable rearrangements within their chromosomes suggesting that both species evolved separately for a relatively long period of time and adapted to specific tasks within microbial consortia responsible for anaerobic digestion.

Keywords
Clostridium; Acidogenic bacterium; Cellulosomes; Megaplasmid; Metabolic pathway reconstruction
First Page Preview
Complete genome analysis of Clostridium bornimense strain M2/40T: A new acidogenic Clostridium species isolated from a mesophilic two-phase laboratory-scale biogas reactor
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 232, 20 August 2016, Pages 38–49
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us