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Hydrogel/calcium phosphate composites require specific properties for three-dimensional culture of human bone mesenchymal cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
2254 107 2010 8 PDF Available
Title
Hydrogel/calcium phosphate composites require specific properties for three-dimensional culture of human bone mesenchymal cells
Abstract

To provide multipotent cells with a three-dimensional environment closer to bone matrix, an engineered construct mimicking bone components has been designed and evaluated. A biocompatible hydrogel (silated hydroxypropylmethyl cellulose) was used as an extra-cellular matrix while biphasic calcium phosphate ceramic particles were used to replace mineralized matrix. Finally, human bone mesenchymal cells were cultured in three dimensions in the resulting constructs to study their cell viability, proliferation, interactions within the composites, and maintenance of their osteogenic potential. This approach resulted in homogeneous structures in which cells were viable and retained their osteoblastic differentiation potential. However, the cells did not proliferate nor colonize the constructs, possibly because of a lack of suitable interactions with their micro-environment.

Keywords
Biomimetics; Hydrogels; Calcium phosphates; Bone repair/regeneration; Stem cells
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Hydrogel/calcium phosphate composites require specific properties for three-dimensional culture of human bone mesenchymal cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 6, Issue 8, August 2010, Pages 2932–2939
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us