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An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host

Paper ID Volume ID Publish Year Pages File Format Full-Text
22541 43366 2016 8 PDF Available
Title
An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host
Abstract

•Phage φC31 attachment sites were integrated into the genome of S. albus J1074.•Secondary metabolite production was tested in a panel of four strains.•Additional copy of secondary metabolite clusters can increase the production up to 700%.•A new compound could be detected, isolated and the structure was elucidated.

Streptomyces albus J1074 is a well-known host for heterologous expression of secondary metabolites. To further increase its potential and to study the influence of cluster multiplication, additional φC31-attachment site was integrated into its genome using a system for transposon mutagenesis. Four secondary metabolite clusters were expressed in strains with different numbers of attachment sites, ranging from one to three copies of the site. Secondary metabolite production was examined and a new compound could be detected, purified and its structure was elucidated.

Keywords
Heterologous expression; Antibiotic production; Strain development; Streptomyces albus J1074; φC31-encoded recombination system; Multiple attachment sites
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An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 232, 20 August 2016, Pages 110–117
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
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