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Production of ω-hydroxy octanoic acid with Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
22648 43368 2016 4 PDF Available
Title
Production of ω-hydroxy octanoic acid with Escherichia coli
Abstract

•E. coli as a bacterial host for the de novo production of mid-chain fatty acids.•Engineering E. coli for the increased production of octanoic acid.•Establishing a whole-cell biocatalyst for the production of ω-hydroxy octanoic acid.

The present proof-of-concept study reports the construction of a whole-cell biocatalyst for the de novo production of ω-hydroxy octanoic acid. This was achieved by hijacking the natural fatty acid cycle and subsequent hydroxylation using a specific monooxygenase without the need for the additional feed of alkene-like precursors. For this, we used the model organism Escherichia coli and increased primarily the release of the octanoic acid precursors by overexpressing the plant thioesterase FatB2 from Cuphea hookeriana in a β-oxidation deficient strain, which lead to the production of 2.32 mM (8.38 mg gcww−1) octanoic acid in 24 h. In order to produce the corresponding ω-hydroxy derivative, we additionally expressed the engineered self-sufficient monooxygenase fusion protein CYP153AMaq(G307A)-CPRBM3 within the octanoic acid producing strain. With this, we finally produced 234 μM (0.95 mg gcww−1) ω-hydroxy octanoic acid in a 20 h fed-batch set-up.

Keywords
Octanoic acid; ω-hydroxy octanoic acid; Thioesterase; Monooxygenase; E. coli; Fatty acids
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 230, 20 July 2016, Pages 30–33
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us