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Construction of recombinant baculovirus vaccines for Newcastle disease virus and an assessment of their immunogenicity

Paper ID Volume ID Publish Year Pages File Format Full-Text
22662 43369 2016 11 PDF Available
Title
Construction of recombinant baculovirus vaccines for Newcastle disease virus and an assessment of their immunogenicity
Abstract

•Newcastle Disease Virus (NDV) is an infectious poultry disease with high mortality.•Baculovirus vaccines were engineered expressing the NDV F and HN proteins.•The F-series was more immunogenic and offered better protection than the HN-series.•WPRE and VSV-GED elements increased vaccine immunogenicity and antigen expression.•Internal terminal repeats (ITRs) increased the duration of the cytokine response.

Newcastle disease (ND) is a lethal avian infectious disease caused by Newcastle disease virus (NDV) which poses a substantial threat to China’s poultry industry. Conventional live vaccines against NDV are available, but they can revert to virulent strains and do not protect against mutant strains of the virus. Therefore, there is a critical unmet need for a novel vaccine that is safe, efficacious, and cost effective. Here, we designed novel recombinant baculovirus vaccines expressing the NDV F or HN genes. To optimize antigen expression, we tested the incorporation of multiple regulatory elements including: (1) truncated vesicular stomatitis virus G protein (VSV-GED), (2) woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), (3) inverted terminal repeats (ITRs) of adeno-associated virus (AAV Serotype II), and (4) the cytomegalovirus (CMV) promoter. To test the in vivo efficacy of the viruses, we vaccinated chickens with each construct and characterized the cellular and humoral immune response to challenge with virulent NDV (F48E9). All of the vaccine constructs provided some level of protection (62.5–100% protection). The F-series of vaccines provided a greater degree of protection (87.5–100%) than the HN-series (62.5–87.5%). While all of the vaccines elicited a robust cellular and humoral response subtle differences in efficacy were observed. The combination of the WPRE and VSV-GED regulatory elements enhanced the immune response and increased antigen expression. The ITRs effectively increased the length of time IFN-γ, IL-2, and IL-4 were expressed in the plasma. The F-series elicited higher titers of neutralizing antibody and NDV-specific IgG. The baculovirus system is a promising platform for NDV vaccine development that combines the immunostimulatory benefits of a recombinant virus vector with the non-replicating benefits of a DNA vaccine.

Keywords
Newcastle disease virus; F gene; HN gene; Baculovirus expression vector system; Immunogenicity
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Construction of recombinant baculovirus vaccines for Newcastle disease virus and an assessment of their immunogenicity
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 231, 10 August 2016, Pages 201–211
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us