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Tobacco Etch Virus protease: A shortcut across biotechnologies

Paper ID Volume ID Publish Year Pages File Format Full-Text
22663 43369 2016 11 PDF Available
Title
Tobacco Etch Virus protease: A shortcut across biotechnologies
Abstract

•TEV protease is the most popular endopeptidase for biotechnological applications.•The unique features of TEV protease are described.•The main in vitro and in vivo applications of TEV protease are reviewed.

About thirty years ago, studies on the RNA genome of Tobacco Etch Virus revealed the presence of an efficient and specific protease, called Tobacco Etch Virus protease (TEVp), that was part of the Nuclear Inclusion a (NIa) enzyme. TEVp is an efficient and specific protease of 27 kDa that has become a valuable biotechnological tool. Nowadays TEVp is a unique endopeptidase largely exploited in biotechnology from industrial applications to in vitro and in vivo cellular studies. A number of TEVp mutants with different rate of cleavage, stability and specificity have been reported. Similarly, a panel of different target cleavage sites, derived from the canonical ENLYFQ-G/S site, has been established. In this review we describe these aspects of TEVp and some of its multiple applications. A particular focus is on the use and molecular biology of TEVp in living cells and organisms.

Keywords
TEV protease; Self-cleavage; In vivo applications; Protein engineering; Split-TEV; Subcellular targeting
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 231, 10 August 2016, Pages 239–249
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us