fulltext.study @t Gmail

Impacts of proline on the central metabolism of an industrial erythromycin-producing strain Saccharopolyspora erythraea via 13C labeling experiments

Paper ID Volume ID Publish Year Pages File Format Full-Text
22665 43369 2016 8 PDF Available
Title
Impacts of proline on the central metabolism of an industrial erythromycin-producing strain Saccharopolyspora erythraea via 13C labeling experiments
Abstract

•Very low growth rate of S. erythraea in minimal medium.•Low activity of EMP pathway and high activity of ED pathway for glucose utilization.•Promotion on cell growth of proline and use of proline as building block.•Proline addition relieved the energy burden for cell growth.

Saccharopolyspora erythraea E3 is an important industrial strain for erythromycin production and knowledge on its metabolism is limited. In the present work, 13C labeling experiments were conducted to characterize the metabolism of S. erythraea E3. We found that S. erythraea E3 was difficult to grow on minimal medium with glucose as sole carbon source and the addition of proline remarkably improved the cell growth. The activity of EMP pathway was very low and ED pathway was alternatively the main glucose utilization pathway. The addition of proline resulted in remarkable changes in the fluxes of central metabolism. The fluxes in PP pathway, in TCA cycle and in ED pathway were 90% higher, 64% and 31% lower on Glc/Pro than on Glc, respectively. The maintenance energy on Glc/Pro was 58.4% lower than that on Glc. The energy charge was lower on Glc than on Glc/Pro, indicating that the cells on Glc suffered from energy burden. This study elucidates the impacts of proline on the central metabolism of S. erythraea and deepens the understanding of its metabolism.

Keywords
FL, fractional labeling; EMP, Embden-Meyerhof-Parnas; ED, Entner-Doudoroff; PP, pentose phosphate; TCA, tricarboxylic acid cycle; MFA, metabolic flux analysis; SSR, sum of the squared residuals; GAP, glyceraldehyde-3-phosphate; G6P, glucose 6- phosphate;
First Page Preview
Impacts of proline on the central metabolism of an industrial erythromycin-producing strain Saccharopolyspora erythraea via 13C labeling experiments
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 231, 10 August 2016, Pages 1–8
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering