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Cocoa pod husk: A new source of CLEA-lipase for preparation of low-cost biodiesel: An optimized process

Paper ID Volume ID Publish Year Pages File Format Full-Text
22669 43369 2016 11 PDF Available
Title
Cocoa pod husk: A new source of CLEA-lipase for preparation of low-cost biodiesel: An optimized process
Abstract

•Biodiesel was produced enzymatically from Jatropha curcas oil and ethanol.•Low-cost CLEA-lipase from cocoa pod husk (CPH) was applied in this study.•Biodiesel production using CLEA-lipase from CPH, is the first biodiesel prepared using a non-microbial source of lipase.•Face centered central composite design (FCCCD) under response surface methodology (RSM) was used to get the optimal conditions of conversion (%) of FFA into biodiesel.

Enzymatic reactions involving lipases as catalyst in transesterification can be an excellent alternative to produce environmental-friendly biodiesel. In this study, lipase extracted from Cocoa Pod Husk (CPH) and immobilized through cross linked enzyme aggregate (CLEA) technology catalysed the transesterification of Jatropha curcas oil successfully. Face centered central composite design (FCCCD) under response surface methodology (RSM) was used to get the optimal conditions of 3% (w/w) enzyme loading, 4 h reaction time and 1:6 oil/ethanol ratio to achieve the highest conversion of free fatty acid and glycerides into biodiesel (93%). The reusability of CLEA-lipase was tested and after seven cycles, the conversion percentage reduced to 58%. The results revealed that CLEA lipase from CPH is a potential catalyst for biodiesel production.

Keywords
CPH, cocoa pod husk; FCCCD, face centered central composite design; RSM, response surface methodology; CLEA, cross-linked enzyme aggregates; FFA, free fatty acids; FAEE, fatty acid ethyl ester; GC/MS, gas chromatography/mass spectrometryCocoa pod husk; CL
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Cocoa pod husk: A new source of CLEA-lipase for preparation of low-cost biodiesel: An optimized process
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 231, 10 August 2016, Pages 95–105
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us