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Recombinant baculovirus mediates dsRNA specific to rr2 delivery and its protective efficacy against WSSV infection

Paper ID Volume ID Publish Year Pages File Format Full-Text
22696 43370 2016 9 PDF Available
Title
Recombinant baculovirus mediates dsRNA specific to rr2 delivery and its protective efficacy against WSSV infection
Abstract

•This work reported the successful construction of recombinant baculovirus displaying VP28 of WSSV and a gene encoding dsRNA specific to rr2.•This recombinant virus could entry to shrimp hemocytes.•The recombinant virus encoding a gene for rr2 dsRNA synthesis highly reduced the cumulative mortality in WSSV infection.•The WSSV copy number was highly reduced in WSSV-infected shrimp injected with recombinant virus encoding a gene for rr2 dsRNA synthesis.•The knockdown of rr2 was also detected in WSSV-infected shrimp injected with recombinant virus encoding a gene for rr2 dsRNA synthesis.

White spot syndrome virus (WSSV) is a major causative agent in shrimp farming. Consequently, RNAi technology is an effective strategy to prevent WSSV infection in shrimp especially dsRNA targeting to rr2 of WSSV. In an effort to develop dsRNA expression in shrimp for control of WSSV infection, we developed a recombinant baculovirus expressing recombinant VP28 as the gene delivery system to carry a gene encoding dsRNA specific to rr2 for triggering the RNAi process in shrimp. The results showed that the recombinant baculovirus harboring VP28 was able to express VP28 indicated by Western blot with polyclonal antibody specific to VP28. VP28 transcript was detected in shrimp hemocytes after co-culture hemocytes with the recombinant baculovirus displaying VP28. In addition, we found that shrimp injected with the recombinant baculovirus displaying VP28 and encoding dsRNA synthetic gene specific to rr2 (Bac-VP28-dsrr2) showed the lowest cumulative mortality (33%) at 14 days post infection (dpi) when compared to shrimp injected with baculovirus displaying VP28 (Bac-VP28) (64% cumulative mortality) (p < 0.05). According to the results, shrimp injected with Bac-VP28-dsrr2 also showed significantly lower WSSV copies than shrimp injected with Bac-VP28 (p < 0.05) along with the down-regulation of rr2 expression at 1, 3 and 7 dpi. In conclusion, the Bac-VP28-dsrr2 was effective in prevention of WSSV infection. Therefore, the results obtained here can be applied to the prevention of WSSV infection by mixing the recombinant baculovirus with shrimp feed in the future.

Keywords
White spot syndrome virus; RNAi technology; VP28; rr2; Baculovirus
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Recombinant baculovirus mediates dsRNA specific to rr2 delivery and its protective efficacy against WSSV infection
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 229, 10 July 2016, Pages 44–52
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us