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Action of three GH51 and one GH54 α-arabinofuranosidases on internally and terminally located arabinofuranosyl branches

Paper ID Volume ID Publish Year Pages File Format Full-Text
22700 43370 2016 9 PDF Available
Title
Action of three GH51 and one GH54 α-arabinofuranosidases on internally and terminally located arabinofuranosyl branches
Abstract

•ABF action was studied against all Araf mono- and disubstitutions in xylan and AXOS.•Three GH51 ABFs removed Araf mono- and disubstitutions from terminal Xylp residues.•GH51 A. niger and GH54 T. reesei ABF degraded Araf disubstitutions on internal Xylp.•GH54 T. reesei ABF favoured O-2-Araf on internal Xylp and O-3-Araf on terminal Xylp.•This change in preference occurred on both mono- and disubstitutions.

The action of three glycoside hydrolase family GH51 and one GH54 α-arabinofuranosidases (ABFs) was studied on polymeric arabinoxylan and arabinoxylooligosaccharides. The substrates covered all possible arabinofuranosyl (Araf) substituents, i.e., terminal and internal α(1 → 2) and α(1 → 3)-linked Araf monosubstitutions and disubstitutions. The GH51 ABFs removed nearly all mono- and disubstitutions from terminal non-reducing end xylopyranosyl (Xylp) residues, showing dual ABF-m/d activity. From internal Xylp, primarily monosubstitutions were removed, except after treatment with GH51 Aspergillus niger ABF. It degraded slowly internal disubstitutions as well, showing versatility in substrate specificity within GH51. GH54 Trichoderma reesei ABF core protein also presented dual ABF-m/d activity, slowly degrading Araf disubstitutions from both terminal and internal positions. Surprisingly, regioselectivity of the T. reesei ABF changed from α(1 → 3)-linked Araf on terminal Xylp to α(1 → 2)-linked Araf on internal Xylp on both mono- and disubstitutions. In conclusion, systematic analysis of natural substrates revealed interesting new details on the action T. reesei ABF and showed that the dual ABF-m/d activity could be more prevalent than previously thought, especially within GH51 ABFs.

Keywords
ABF, α-arabinofuranosidase; Araf, arabinofuranosyl; AXOS, arabinoxylooligosaccharides; GH, glycoside hydrolase; HPAEC-PAD, high-performance anion exchange chromatography with pulsed amperometric detection; WAX-LV, low-viscosity wheat flour arabinoxylan; X
First Page Preview
Action of three GH51 and one GH54 α-arabinofuranosidases on internally and terminally located arabinofuranosyl branches
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 229, 10 July 2016, Pages 22–30
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering