fulltext.study @t Gmail

Conjugative transfer of broad host range plasmids to an acidobacterial strain, Edaphobacter aggregans

Paper ID Volume ID Publish Year Pages File Format Full-Text
22709 43373 2016 7 PDF Available
Title
Conjugative transfer of broad host range plasmids to an acidobacterial strain, Edaphobacter aggregans
Abstract

•Conjugative transfer and stability of plasmids to an acidobacterium were studied.•Plasmid pMOL98 was more stable than pKT230 in .•PromA and IncQ plasmid host range is extended for the first time to acidobacteria

The Acidobacteria phylum is of high ecological interest. Its members are ubiquitous and particularly abundant in soils but many are recalcitrant to cultivation in the laboratory. Thus, the ability of Acidobacteria to capture and maintain plasmids remains largely unexplored. In this work we tested the transfer and the stability of (i) the PromA plasmid pMOL98 and (ii) the IncQ plasmid pKT230 to the acidobacterial strain Edaphobacter aggregans DSM 19364. To this end quantitative conjugation assays were performed and transconjugants were scored for plasmid-borne antibiotic selection markers. The tested plasmids were transferred and maintained in the new host. Plasmid pMOL98 was more stable than pKT230 in Ed. aggregans in the absence of positive selection. Thus, from an ecological point of view, we have extended the host range of PromA and IncQ plasmids for the first time to an acidobacterial strain. Furthermore, we have uncovered the potential of Acidobacteria to capture as-yet-unknown plasmids and to foster the development of new cloning and expression systems for the exploitation of biotechnologically valuable soil resources.

Keywords
Acidobacteria; Conjugation; Broad host range plasmids; Plasmid stability/transfer
First Page Preview
Conjugative transfer of broad host range plasmids to an acidobacterial strain, Edaphobacter aggregans
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 221, 10 March 2016, Pages 107–113
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us