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Selective oxidation of trimethylolpropane to 2,2-bis(hydroxymethyl)butyric acid using growing cells of Corynebacterium sp. ATCC 21245

Paper ID Volume ID Publish Year Pages File Format Full-Text
22713 43373 2016 8 PDF Available
Title
Selective oxidation of trimethylolpropane to 2,2-bis(hydroxymethyl)butyric acid using growing cells of Corynebacterium sp. ATCC 21245
Abstract

•Conversion of polyols to hydroxycarboxylic acid by whole cells biotransformation.•Selective oxidation of trimethylolpropane (TMP) using Corynebacterium sp.•Enhancement of oxidation in presence of co-substrates.•pH 8 and 1 v/v/m airflow are optimal conditions for the oxidation process.•Product recovery using ion exchange resin.

Multifunctional chemicals including hydroxycarboxylic acids are gaining increasing interest due to their growing applications in the polymer industry. One approach for their production is a biological selective oxidation of polyols, which is difficult to achieve by conventional chemical catalysis. In the present study, trimethylolpropane (TMP), a trihydric alcohol, was subjected to selective oxidation using growing cells of Corynebacterium sp. ATCC 21245 as a biocatalyst and yielding the dihydroxy-monocarboxylic acid, 2,2-bis(hydroxymethyl)butyric acid (BHMB). The study revealed that co-substrates are crucial for this reaction. Among the different evaluated co-substrates, a mixture of glucose, xylose and acetate at a ratio of 5:5:2 was found optimum. The optimal conditions for biotransformation were pH 8, 1 v/v/m airflow and 500 rpm stirring speed. In batch mode of operation, 70.6% of 5 g/l TMP was converted to BHMB in 10 days. For recovery of the product the adsorption pattern of BHMB to the anion exchange resin, Ambersep® 900 (OH−), was investigated in batch and column experiments giving maximum static and dynamic binding capacities of 135 and 144 mg/g resin, respectively. BHMB was separated with 89.7% of recovery yield from the fermentation broth. The approach is applicable for selective oxidation of other highly branched polyols by biotransformation.

Keywords
Selective oxidation; Corynebacterium sp.; Trimethylolpropane; 2,2-Bis(hydroxymethyl)butyric acid; Whole cell biotransformation; Product recovery
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Selective oxidation of trimethylolpropane to 2,2-bis(hydroxymethyl)butyric acid using growing cells of Corynebacterium sp. ATCC 21245
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 221, 10 March 2016, Pages 62–69
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us