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Design and construction of a new human naïve single-chain fragment variable antibody library, IORISS1

Paper ID Volume ID Publish Year Pages File Format Full-Text
22726 43374 2016 11 PDF Available
Title
Design and construction of a new human naïve single-chain fragment variable antibody library, IORISS1
Abstract

•Design and construction of a new human naïve antibody phage display library IORISS1.•Optimized method used to generate a new human naïve phage library.•Diversity of V genes obtained by careful amplification.

Human monoclonal antibodies are a powerful tool with increasingly successful exploitations and the single chain fragment variable format can be considered the building block for the implementation of more complex and effective antibody-based constructs. Phage display is one of the best and most efficient methods to isolate human antibodies selected from an efficient and variable phage display library. We report a method for the construction of a human naïve single-chain variable fragment library, termed IORISS1. Many different sets of oligonucleotide primers as well as optimized electroporation and ligation reactions were used to generate this library of 1.2 × 109 individual clones. The key difference is the diversity of variable gene templates, which was derived from only 15 non-immunized human donors. The method described here, was used to make a new human naïve single-chain fragment variable phage display library that represents a valuable source of diverse antibodies that can be used as research reagents or as a starting point for the development of therapeutics. Using biopanning, we determined the ability of IORISS1 to yield antibodies. The results we obtained suggest that, by using an optimized protocol, an efficient phage antibody library can be generated.

Keywords
Biopanning; IORISS1; Monoclonal antibody; Naïve library single-chain variable fragment; Phage display
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Design and construction of a new human naïve single-chain fragment variable antibody library, IORISS1
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 224, 20 April 2016, Pages 1–11
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us