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Engineering an Escherichia coli platform to synthesize designer biodiesels

Paper ID Volume ID Publish Year Pages File Format Full-Text
22729 43374 2016 8 PDF Available
Title
Engineering an Escherichia coli platform to synthesize designer biodiesels
Abstract

•Develop an E. coli platform to synthesize biodiesels with tunable fatty acid and alcohol moieties.•Design fatty acid ethyl and isobutyl ester pathways as exchangeable biodiesel production modules.•Demonstrate an E. coli modular chassis carrying biodiesel modules enhanced biodiesel production.•Demonstrate biodiesel modules can be mixed to control alcohol moieties of designer biodiesels.

Biodiesels, fatty acid esters (FAEs), can be synthesized by condensation of fatty acid acyl CoAs and alcohols via a wax ester synthase in living cells. Biodiesels have advantageous characteristics over petrodiesels such as biodegradability, a higher flash point, and less emission. Controlling fatty acid and alcohol moieties are critical to produce designer biodiesels with desirable physiochemical properties (e.g., high cetane number, low kinematic viscosity, high oxidative stability, and low cloud point). Here, we developed a flexible framework to engineer Escherichia coli cell factories to synthesize designer biodiesels directly from fermentable sugars. In this framework, we designed each FAE pathway as a biodiesel exchangeable production module consisting of acyl CoA, alcohol, and wax ester synthase submodules. By inserting the FAE modules in an engineered E. coli modular chassis cell, we generated E. coli cell factories to produce targeted biodiesels (e.g., fatty acid ethyl (FAEE) and isobutyl (FAIbE) esters) with tunable and controllable short-chain alcohol moieties. The engineered E. coli chassis carrying the FAIbE production module produced 54 mg/L FAIbEs with high specificity, accounting for >90% of the total synthesized FAEs and ∼4.7 fold increase in FAIbE production compared to the wildtype. Fed-batch cultures further improved FAIbE production up to 165 mg/L. By mixing ethanol and isobutanol submodules, we demonstrated controllable production of mixed FAEEs and FAIbEs. We envision the developed framework offers a flexible, alternative route to engineer designer biodiesels with tunable and controllable properties using biomass-derived fermentable sugars.

Keywords
Fatty acid ethyl esters; Fatty acid isobutyl esters; Advanced biofuels; Designer biodiesels; Modular cell chassis; Biodiesel modules
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Engineering an Escherichia coli platform to synthesize designer biodiesels
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 224, 20 April 2016, Pages 27–34
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us