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Microbiome response to controlled shifts in ammonium and LCFA levels in co-digestion systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
22746 43380 2016 10 PDF Available
Title
Microbiome response to controlled shifts in ammonium and LCFA levels in co-digestion systems
Abstract

•Gradual changes in environmental conditions allow bacterial communities adaptation.•Archaeal communities do not adapt to increasing ammonium or LCFA levels.•Pseudomonadaceae, Carnobacteriaceae and Clostridiadaceae resist high ammonia levels.•Anaerobaculaceae family confronts high lipid concentrations.

Anaerobic co-digestion using protein-rich and lipid-rich co-substrates is limited by the accumulation of ammonia and long chain fatty acids (LCFAs), which are important inhibitors of the anaerobic microorganisms. This work aimed to study the microbial community dynamics during gradual and abrupt increase in ammonium and LCFAs concentrations by applying several molecular techniques, as well as during gradual decrease. For this purpose, two anaerobic reactors co-digesting three agro-industrial wastes underwent abrupt and gradual changes of ammonium and LCFAs concentrations. Both variations provoked volatile fatty acids (VFAs) accumulation, mainly acetic acid up to 4.5 g L−1. High ammonium levels were correlated to an increase in Pseudomonadaceae, Carnobacteriaceae and Clostridiadaceae families and to a drop in Syntrophomonadaceae. However, high LCFA levels provoked an increase in the Anaerobaculaceae and Peptococcaceae families. Both perturbations resulted in greater variations in the archaeal domain, going from Methanosaeta dominance in steady state to hydrogenotrophic pathway during the disturbance periods. During the abrupt changes, Bacteria domain experienced a minimal change, which indicates the adaptation bacterial populations to high ammonium and LCFAs levels. Species belonging to Porphyromonadaceae and Tissierellaceae families linked to VFAs consumption rose their presence during the recovery period. This study identifies a subset of microbial communities linked to high ammonia and LCFA concentrations, useful for optimizing the high-rate co-digestion processes dealing with lipid and protein-rich co-substrates.

Keywords
COD, chemical oxygen demand; db-RDA, constrained distance-based redundancy analysis; DGGE, denaturing gradient gel electrophoresis; FISH, fluorescent in situ hybridization; FW, fish waste; HRT, hydraulic retention time; LCFA, long chain fatty acids; MR, b
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Microbiome response to controlled shifts in ammonium and LCFA levels in co-digestion systems
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 220, 20 February 2016, Pages 35–44
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us