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An insight on acyl migration in solvent-free ethanolysis of model triglycerides using Novozym 435

Paper ID Volume ID Publish Year Pages File Format Full-Text
22747 43380 2016 8 PDF Available
Title
An insight on acyl migration in solvent-free ethanolysis of model triglycerides using Novozym 435
Abstract

•Ethanolysis of S, M and LCFA triglycerides (TG) catalyzed by Novozym 435.•Focusing on the secondary reaction of acyl migration.•2-Monoglyceride was favored by long reaction times and large biocatalyst loading with saturated S and MCFA TG.•Acyl migration increased the yield of ethyl esters and minimized the content of 2-monoglyceride.•Conversion of acylglycerides with unsaturated LCFA was low due to limitations in their access to the active site of the lipase.

In this work, the ethanolysis of triglycerides catalyzed by immobilized lipase was studied, focusing on the secondary reaction of acyl migration. The catalytic tests were performed in a solvent-free reaction medium using Novozym 435 as biocatalyst.The selected experimental variables were biocatalyst loading (5–20 mg), reaction time (30–90 min), and chain length of the fatty acids in triglycerides with and without unsaturation (short (triacetin), medium (tricaprylin) and long (tripalmitin/triolein)).The formation of 2-monoglyceride by ethanolysis of triglycerides was favored by long reaction times and large biocatalyst loading with saturated short- to medium-chain triglycerides. In the case of long-chain triglycerides, the formation of this monoglyceride was widely limited by acyl migration.In turn, acyl migration increased the yield of ethyl esters and minimized the content of monoglycerides and diglycerides. Thus, the enzymatic synthesis of biodiesel was favored by long-chain triglycerides (which favor the acyl migration), long reaction times and large biocatalyst loading.The conversion of acylglycerides made from long-chain fatty acids with unsaturation was relatively low due to limitations in their access to the active site of the lipase.

Keywords
1,2-DAG, 1,2-diacylglyceride(s); 1,3-DAG, 1,3-diacylglyceride(s); 1-MAG, 1-monoacylglyceride(s); 2,3-DAG, 2,3-diacylglyceride(s); 2-MAG, 2-monoacylgliceride(s); 3-MAG, 3-monoacylglyceride(s); AM, acyl migration; CALB, Candida antarctica lipase B; ED1, Exp
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An insight on acyl migration in solvent-free ethanolysis of model triglycerides using Novozym 435
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 220, 20 February 2016, Pages 92–99
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us