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Production of Escherichia coli-based virus-like particle vaccine against porcine circovirus type 2 challenge in piglets: Structure characterization and protective efficacy validation

Paper ID Volume ID Publish Year Pages File Format Full-Text
22761 43381 2016 5 PDF Available
Title
Production of Escherichia coli-based virus-like particle vaccine against porcine circovirus type 2 challenge in piglets: Structure characterization and protective efficacy validation
Abstract

•Soluble non-tag full-length PCV2 capsid protein (rCap) was produced in Escherichia coli and self-assembled into VLP in vitro.•The Cryo-EM structure of PCV2 rCap VLP was determined to 4.5 Å resolution.•PCV2 rCap VLP-based vaccine displayed strong protective efficacy against PCV2 challenge as a commercial vaccine.

We report the strategies leading to the large-production of soluble non-tag full-length porcine circovirus type 2 (PCV2) Cap protein in Escherichia coli. Under neutral pH condition, the purified recombinant Cap protein derived from E. coli expression self-assembles into homogenous round virus-like particle at the similar size of that of the intact PCV2 virus, which is further characterized by Cryo-EM single particle structure determined at 4.5 Å. The engineered PCV2 rCap VLP was tested as a subunit vaccine for the protective efficacy against PCV2 challenge on 3-week old piglets. Similar to commercial available PCV2 vaccine, the Cap VLP-immunized piglets developed specific antibody-mediated response and were protected from the virulent SH PCV2 strain challenge. Hence, the production of E. coli based PCV2Cap-VLP could be applied as a cost-friendly and effective subunit vaccine to control PCV2 spreading in developing countries.

Keywords
Porcine circovirus type 2; Escherichia coli expression; Virus-like particle; Cryo-EM structure; Vaccine efficacy
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Production of Escherichia coli-based virus-like particle vaccine against porcine circovirus type 2 challenge in piglets: Structure characterization and protective efficacy validation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 223, 10 April 2016, Pages 8–12
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us