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Diffusional and transcriptional mechanisms involved in laccases production by Pleurotus ostreatus CP50

Paper ID Volume ID Publish Year Pages File Format Full-Text
22764 43381 2016 8 PDF Available
Title
Diffusional and transcriptional mechanisms involved in laccases production by Pleurotus ostreatus CP50
Abstract

•Opposite effects of EDCF and DOT on fungal physiology were observed.•DOT above 8% negatively affects poxc transcription and laccase specific production.•EDCF positively affects fungal growth and laccase specific production.•Cultures <11 kW/m3s EDCF produce pellets with diffusional limitations.•DOT effects were transcriptional whereas EDCF effects were mainly physical.

The independent effects of hydrodynamic stress (assessed as the Energy Dissipation/Circulation Function, EDCF) and dissolved oxygen tension (DOT) on the growth, morphology and laccase production by Pleurotus ostreatus CP50 were studied using a 32 factorial design in a 10 L reactor. A bell-shape function for fungus growth between 8 and 22% DOT was observed, as well as a significant negative effect on laccase production and the expression of poxc, the gene encoding for the most abundant laccase produced by P. ostreatus CP50. Increasing EDCF from 1 to 21 kW/m3 s, had a positive effect on fungus growth, whereas no effect on poxc gene expression was observed. However, the increase in EDCF favored the specific laccase production due to the generation of smaller pellets with less diffusional limitations and increased metabolically active biomass.The results show, for the first time, that hydrodynamic effects on growth and laccase production are mainly physical and diffusional, while the influence of the dissolved oxygen is at transcriptional level.

Keywords
Hydrodynamics; Dissolved oxygen; Gene expression; Laccases; Pleurotus ostreatus.
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Diffusional and transcriptional mechanisms involved in laccases production by Pleurotus ostreatus CP50
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 223, 10 April 2016, Pages 42–49
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us