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Eri silkworm (Samia ricini), a non-mulberry host system for AcMNPV mediated expression of recombinant proteins

Paper ID Volume ID Publish Year Pages File Format Full-Text
22778 43384 2015 6 PDF Available
Title
Eri silkworm (Samia ricini), a non-mulberry host system for AcMNPV mediated expression of recombinant proteins
Abstract

•AcMNP baculovirus replicates in Eri silkworm larvae leading to productive infection.•Upon intrahaemocoelical inoculation of AcMNPV carrying GFP reporter gene, virus localization was evidenced in various tissues.•Eri silkworm insect larva serves as mini biofactory as shown by the expression of FMDV protein 3ABC.•The Eri silkworm larval system could be used as a new low cost platform for scaling up of recombinant proteins.

The baculovirus expression system (BVES) based on Autographa californica nucleopolyhedrovirus (AcMNPV) is widely used for the expression of eukaryotic proteins. Several insect cells/larvae that are permissive to AcMNPV have been routinely used as hosts to express heterologous proteins. Domesticated Eri silkworm (Samia ricini), reared in many parts of India, Japan and China, is a non-mulberry silkworm. The present study shows that the Eri silkworm larvae are susceptible to intra-haemocoelical inoculation of AcMNPV. The virus replicates in the larva, as indicated by an increased viral loads in the haemolymph upon injection of a recombinant AcMNPV carrying green fluorescent protein gene. The virus showed localized replication in different tissues including the fat body, haemocytes, tracheal matrix and in the Malphigian tubules. The larval system was successfully used to express heterologous protein, by infecting with a recombinant AcMNPV carrying the 3ABC coding sequence of foot-and-mouth disease virus (FMDV). The study shows that the Eri silkworm larva can be a potential alternative bioreactor, for scaling up of the recombinant proteins employing the baculovirus system.

Keywords
Baculovirus; AcMNPV; Eri silkworm (Samia ricini); Infection; Bioreactor; FMDV 3ABC
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Eri silkworm (Samia ricini), a non-mulberry host system for AcMNPV mediated expression of recombinant proteins
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 216, 20 December 2015, Pages 76–81
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us