fulltext.study @t Gmail

Evaluation of various techniques for microalgal biomass quantification

Paper ID Volume ID Publish Year Pages File Format Full-Text
22779 43384 2015 8 PDF Available
Title
Evaluation of various techniques for microalgal biomass quantification
Abstract

•Biomass quantification of microalgae using new techniques.•Dynamic ranges of measurements higher than 20 g l-1 by dielectric permittivity and ODNIR sensors.•Oxygen production rate is a good proxy of microalgae concentration in DCW lower than 1 g l-1.•Fourier equation enhanced the image analysis for estimation of microalgae concentration up to 6 g l-1.

Biomass concentration is one of the most important parameters in the biotechnology processes. Its measurement relies on the physical, chemical or biological properties of the cells. Several techniques were applied in this work to measure the cell concentration of four microalgae: Botryococcussp., Botryococcusbraunii, Chlorella vulgaris, and Ettlia sp. The experiments were performed using samples taken from a chemostat for each strain to provide microalgal cell suspensions in a stable physiological state and concentration. The dry cell weight (DCW) was used as the reference method for the evaluation of other methods. The two commercial sensors used to determine optical density and dielectric permittivity showed a broad effective measurement range up to more than 20 g l−1. A Red–Green–Blue model analysis of microalgal digital images in combination with Fourier equation significantly extended the measurements range up to 6 g l−1. Cell count using a flow cytometer showed a broad range of linearity to DCW in washed samples, but other counting methods using hemocytometer and microscopic automated count were limited. Finally, the oxygen production rate, representing the photosynthetic activity, showed a linear regression with DCW at cell concentrations lower than 1 g l−1.

Keywords
Dielectric permittivity; Near infrared optical density; Oxygen production rate; RGB color model; Biofuels
First Page Preview
Evaluation of various techniques for microalgal biomass quantification
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 216, 20 December 2015, Pages 90–97
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us