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High-level production of Bacillus cereus phospholipase C in Corynebacterium glutamicum

Paper ID Volume ID Publish Year Pages File Format Full-Text
22783 43384 2015 7 PDF Available
Title
High-level production of Bacillus cereus phospholipase C in Corynebacterium glutamicum
Abstract

•A rapid genetic engineering platform was used to evaluate Sec and Tat systems for the secretion of B. Cereus PLC in C. glutamicum to obtain an efficient recombinant strain.•A fed-batch fermentation process was developed to obtain a final concentration of 5.5 g/L of recombinant PLC.•The recombinant BC-PLC shows a high efficiency for enzymatic oil degumming.

Enzymatic oil degumming (removal of phospholipids) using phospholipase C (PLC) is a well-established and environmentally friendly process for vegetable oil refining. In this work, we report the production of recombinant Bacillus cereus PLC in Corynebacterium glutamicum ATCC 13869 in a high cell density fermentation process and its performance in soybean oil degumming. A final concentration of 5.5 g/L of the recombinant enzyme was achieved when the respective gene was expressed from the tac promoter in a semi-defined medium. After treatment with trypsin to cleave the propeptide, the mature enzyme completely hydrolyzed phosphatidylcholine and phosphatidylethanolamine, which represent 70% of the phospholipids present in soybean oil. The results presented here show the feasibility of using B. cereus PLC for oil degumming and provide a manufacturing process for the cost effective production of this enzyme.

Keywords
Synthetic biology; Green chemistry; Enzymatic degumming; Corynebacterium glutamicum; Phospholipase C
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 216, 20 December 2015, Pages 142–148
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us