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Driving carbon flux through exogenous butyryl-CoA: Acetate CoA-transferase to produce butyric acid at high titer in Thermobifida fusca

Paper ID Volume ID Publish Year Pages File Format Full-Text
22784 43384 2015 7 PDF Available
Title
Driving carbon flux through exogenous butyryl-CoA: Acetate CoA-transferase to produce butyric acid at high titer in Thermobifida fusca
Abstract

•An exogenous butyryl-CoA: acetate CoA-transferase gene (actA) was integrated into the chromosome of T. fusca.•33.2 g/L butyric acid was produced from 90.5 g/L cellulose by T. fusca muS-1.•70.4 g/L butyric acid was produced by fed-batch fermentation T. fusca muS-1.•T. fusca muS-1 had the highest butyrate titer 17.1 g/L on corn stover.

Butyric acid, a 4-carbon short chain fatty acid, is widely used in chemical, food, and pharmaceutical industries. The low activity of butyryl-CoA: acetate CoA-transferase in Thermobifida fusca muS, a thermophilic actinobacterium whose optimal temperature was 55 °C, was found to hinder the accumulation of high yield of butyric acid. In order to solve this problem, an exogenous butyryl-CoA: acetate CoA-transferase gene (actA) from Thermoanaerobacterium thermosaccharolyticum DSM571 was integrated into the chromosome of T. fusca muS by replacing celR gene, forming T. fusca muS-1. We demonstrated that on 5 g/L cellulose, the yield of butyric acid by the engineered muS-1 strain was increased by 42.9 % compared to the muS strain. On 100 g/L of cellulose, the muS-1 strain could consume 90.5% of total cellulose in 144 h, with 33.2 g/L butyric acid produced. Furthermore, on the mix substrates including the major components of biomass: cellulose, xylose, mannose and galactose, 70.4 g/L butyric acid was produced in 168 h by fed-batch fermentation. To validate the ability of fermenting biomass, the muS-1 strain was grown on the milled corn stover ranging from 200 to 250 μm. The muS-1 strain had the highest butyrate titer 17.1 g/L on 90 g/L corn stover.

Keywords
Butyric acid; Butyryl-CoAacetate CoA-transferase; Chromosome integration; Metabolic engineering
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Driving carbon flux through exogenous butyryl-CoA: Acetate CoA-transferase to produce butyric acid at high titer in Thermobifida fusca
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 216, 20 December 2015, Pages 151–157
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us