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Emulating human microcapillaries in a multi-organ-chip platform

Paper ID Volume ID Publish Year Pages File Format Full-Text
22788 43384 2015 10 PDF Available
Title
Emulating human microcapillaries in a multi-organ-chip platform
Abstract

•Establishment of a vascular network in a perfused multi-organ-chip.•Optimal fibrinogen concentrations for microvessel formation.•Evidence for self-maintenance of co-cultures in basal medium conditions.

Current microfluidic chip-based tissue culture systems lack a capillary endothelial vessel system, which would enable perfusion with blood. We utilise spatial cell cultures to populate a perfused multi-organ-chip platform—a microfluidic device recently introduced for substance testing. Complete biological vascularization of such culture systems is vital to properly emulate physiological tissue behaviour. In this study, we incorporated a fibrin scaffold into the two-organ-chip design. Herein, adipose-derived stromal cells (ASCs) directed human umbilical vein endothelial cells (HUVECs) to organise into tube-like structures. The ASCs induced tube formation of HUVECs in static and dynamic conditions. The replacement of full medium enriched with growth factors and foetal calf serum with basal medium resulted in viable cells with similar gene expression profiles. We regard this as a prerequisite for studies with organ constructs that have a need for a different medium formulation. Furthermore, we here address stability issues of the fibrin gel and fibrin composition for optimal microvessel formation.

Keywords
2OC, two-organ-chip; 3D, three-dimensional; ACTB, β-actin; ASCs, adipose-derived stromal cells; CD31, cluster of differentiation 31 (also PECAM-1); COX-2, cyclooxygenase-2; eNOS, endothelial nitric oxide synthase; GAPDH, glyceraldehyde 3-phosphate dehydro
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Emulating human microcapillaries in a multi-organ-chip platform
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 216, 20 December 2015, Pages 1–10
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us