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Construction of CoA-dependent 1-butanol synthetic pathway functions under aerobic conditions in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
22803 43392 2015 8 PDF Available
Title
Construction of CoA-dependent 1-butanol synthetic pathway functions under aerobic conditions in Escherichia coli
Abstract

•We design a novel 1-butanol synthetic pathway functions under aerobic conditions.•Engineered E. coli aerobically produces 4.07 g L−1 1-butanol in shaking flask.•Engineered E. coli aerobically produces 8.60 g L−1 1-butanol in a bioreactor.

1-Butanol is an important industrial platform chemical and an advanced biofuel. While various groups have attempted to construct synthetic pathways for 1-butanol production, efforts to construct a pathway that functions under aerobic conditions have met with limited success. Here, we constructed a CoA-dependent 1-butanol synthetic pathway that functions under aerobic conditions in Escherichia coli, by expanding the previously reported (R)-1,3-butanediol synthetic pathway. The pathway consists of phaA (acetyltransferase) and phaB (NADPH-dependent acetoacetyl-CoA reductase) from Ralstonia eutropha, phaJ ((R)-specific enoyl-CoA hydratase) from Aeromonas caviae, ter (trans-enoyl-CoA reductase) from Treponema denticola, bld (butylraldehyde dehydrogenase) from Clostridium saccharoperbutylacetonicum, and inherent alcohol dehydrogenase(s) from E. coli. To evaluate the potential of this pathway for 1-butanol production, culture conditions, including volumetric oxygen transfer coefficient (kLa) and pH were optimized in a mini-jar fermenter. Under optimal conditions, 1-butanol was produced at a concentration of up to 8.60 g L−1 after 46 h of fed-batch cultivation.

Keywords
1-Butanol; Synthetic pathway; E. coli; Aerobic synthesis
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Construction of CoA-dependent 1-butanol synthetic pathway functions under aerobic conditions in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 204, 20 June 2015, Pages 25–32
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us