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Control of specific carbon dioxide production in a fed-batch culture producing recombinant protein using a soft sensor

Paper ID Volume ID Publish Year Pages File Format Full-Text
22919 43394 2015 8 PDF Available
Title
Control of specific carbon dioxide production in a fed-batch culture producing recombinant protein using a soft sensor
Abstract

The feeding of a fed-batch cultivation producing recombinant protein was controlled by a soft sensor set-up. It was assumed that the control approach could be based on the cell's production of carbon dioxide and that this parameter indicates the metabolic state occurring at induced protein expression. The soft sensor used the on-line signals from a carbon dioxide analyser and a near-infrared (NIR) probe for biomass to estimate the specific production rate (qCO2)(qCO2).Control experiments were carried out with various qCO2qCO2 set-points where we observe that the feeding of nutrients to the culture could easily be controlled and resulted in a decreased variability compared to uncontrolled cultivations.We therefore suggest that this control approach could serve as an alternative to other commonly applied methods such as controlling the cell's overflow metabolism of acetate or the cell's specific growth rate. However, further studies of the internal metabolic fluxes of CO2 during protein expression would be recommended for a more precise characterization of the relationship between qCO2qCO2 and protein expression in order to fully interpret the control behaviour.

Keywords
Reproducibility; Variance; Carbon dioxide production rate; Bioprocess regulation; Monitoring; Software sensor
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Control of specific carbon dioxide production in a fed-batch culture producing recombinant protein using a soft sensor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 200, 20 April 2015, Pages 44–51
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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Any Questions? feel free to contact us