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Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools

Paper ID Volume ID Publish Year Pages File Format Full-Text
22921 43394 2015 9 PDF Available
Title
Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools
Abstract

Heterogeneous populations of stably transfected cells (cell pools) can serve for the rapid production of moderate amounts of recombinant proteins. Here, we propose the use of the piggyBac (PB) transposon system to improve the productivity and long-term stability of cell pools derived from Chinese hamster ovary (CHO) cells. PB is a naturally occurring genetic element that has been engineered to facilitate the integration of a transgene into the genome of the host cell. In this report PB-derived cell pools were generated after 10 days of selection with puromycin. The resulting cell pools had volumetric productivities that were 3–4 times higher than those achieved with cell pools generated by conventional plasmid transfection even though the number of integrated transgene copies per cell was similar in the two populations. In 14-day batch cultures, protein levels up to 600 and 800 mg/L were obtained for an Fc-fusion protein and a monoclonal antibody, respectively, at volumetric scales up to 1 L. In general, the volumetric protein yield from cell pools remained constant for up to 3 months in the absence of selection. In conclusion, transfection of CHO cells with the PB transposon system is a simple, efficient, and reproducible approach to the generation of cell pools for the rapid production of recombinant proteins.

Keywords
Recombinant protein; piggyBac; Transposon; Cell pool; Transfection; CHO cells
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Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 200, 20 April 2015, Pages 61–69
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us