fulltext.study @t Gmail

High-yield production of a functional bacteriophage lysin with antipneumococcal activity using a plant virus-based expression system

Paper ID Volume ID Publish Year Pages File Format Full-Text
22922 43394 2015 7 PDF Available
Title
High-yield production of a functional bacteriophage lysin with antipneumococcal activity using a plant virus-based expression system
Abstract

•S. pneumoniae phage lysin Pal was produced in a plant virus-based expression system.•Highly pure Pal was obtained after purification by DEAE sepharose.•Plant-produced Pal was active against three tested S. pneumoniae strains.•Pal treatment of bacteremic mice demonstrated a decrease in blood bacterial titers.•Pal protected mice from streptococcal sepsis and death in a dose responsive manner.

Streptococcus pneumoniae is the causative agent of several serious infectious diseases. It is becoming increasingly antibiotic resistant worldwide, and thus new antimicrobials are needed. One alternative to antibiotics may be the use of peptidoglycan hydrolases, the bacteriophage lytic enzymes.In this study, we demonstrated high level expression of the S. pneumoniae bacteriophage lysin Pal in Nicotiana benthamiana – TMV (Tobacco Mosaic Virus) transient expression system. The protein was purified to homogeneity and tested for streptococci killing activity in vitro and in vivo. In vitro, Pal was able to lyse three tested S. pneumoniae strains: NCTC12695, NCTC12977 and NCTC11888. The treatment of BALB/c mice with 100 μg, 200 μg and 400 μg of Pal 1 h post-challenge with double lethal dose of S. pneumoniae NCTC12695 strain showed a clear dose response and protected from lethal sepsis 30%, 40% and 50% of mice, respectively. The improved mice survival correlated with decreased blood bacterial titers. In conclusion, these results suggest that plant-expressed bacteriophage lysins may have potential use as antimicrobial agents.

Keywords
Plant virus-based transient expression; Bacteriophage lysin; Streptococcus pneumoniae; Mice bacteremia and survival
First Page Preview
High-yield production of a functional bacteriophage lysin with antipneumococcal activity using a plant virus-based expression system
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 200, 20 April 2015, Pages 10–16
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us