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Synthetic polymers are more effective than natural flocculants for the clarification of tobacco leaf extracts

Paper ID Volume ID Publish Year Pages File Format Full-Text
22937 43401 2015 6 PDF Available
Title
Synthetic polymers are more effective than natural flocculants for the clarification of tobacco leaf extracts
Abstract

•Synthetic flocculants can be replaced by non-toxic, natural counterparts.•A combination of chitosan and polyphosphate was the most effective natural flocculant.•A polyethylenimine was the most effective flocculant.•Protein yields were not affected by flocculation.•Complex factor interactions were characterized by design of experiments.

The use of synthetic polymers as flocculants can increase filter capacity and thus reduce the costs of downstream processing during the production of plant-derived biopharmaceutical proteins, but this may also attract regulatory scrutiny due to the potential toxicity of such compounds. Therefore, we investigated the efficacy of three non-toxic natural flocculants (chitosan, kaolin and polyphosphate) alone and in combination with each other or with a synthetic polymer (Polymin P) during the clarification of tobacco leaf extracts. We used a design-of-experiments approach to determine the impact of each combination on filter capacity. We found that Polymin P was most effective when used on its own but the natural flocculants were more effective when used in combination. The combination of chitosan and polyphosphate was the most effective natural flocculant, and this was identified as a potential replacement for Polymin P under neutral and acidic extraction conditions independent of the conductivity, even though the efficiency of flocculation was lower than for Polymin P. None of the tested flocculants reduced the concentration of total soluble protein in the feed stream or the recovery of the model fluorescent protein DsRed.

Keywords
DoE, design of experiments; DSP, downstream processing; LDS-PAGE, lithium dodecylsulfate polyacrylamide gel electrophoresis; NTU, nephelometric turbidity unit; pDADMAC, poly-diallyldimethylammonium chloride; TSP, total soluble proteinBioprocess developmen
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Synthetic polymers are more effective than natural flocculants for the clarification of tobacco leaf extracts
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 195, 10 February 2015, Pages 37–42
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us