fulltext.study @t Gmail

Photosynthetic production of itaconic acid in Synechocystis sp. PCC6803

Paper ID Volume ID Publish Year Pages File Format Full-Text
22938 43401 2015 3 PDF Available
Title
Photosynthetic production of itaconic acid in Synechocystis sp. PCC6803
Abstract

•Itaconic acid was photosynthetically produced in Synechocystis sp. PCC6803.•The optimal dose for IPTG induction was determined using expression vector pJAK12.•Bubbling with CO2 improved itaconic acid production in PCC6803.

Here, we report the photosynthetic production of itaconic acid (IA), a promising building block, from carbon dioxide (CO2) by Synechocystis sp. PCC6803. The engineered PCC6803 strain expressing cis-aconitate decarboxylase, the key enzyme in IA biosynthesis, produced 0.9 mg/L and 14.5 mg/L of IA at production rates of 42.8 μg L−1 day−1 and 919.0 μg L−1 day−1, under conditions of constant bubbling with air and 5% CO2, respectively. This is the first report on the possibility of IA production from CO2 via the photosynthetic process in cyanobacteria.

Keywords
IA, itaconic acid; PCC6803, Synechocystis sp. PCC6803; CAD, cis-aconitate decarboxylase; GFP, green fluorescence protein; CO2, carbon dioxide; IPTG, isopropyl β-d-1-thiogalactopyranoside; LED, light-emitting diodeItaconic acid; Synechocystis sp. PCC6803;
First Page Preview
Photosynthetic production of itaconic acid in Synechocystis sp. PCC6803
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 195, 10 February 2015, Pages 43–45
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us