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Engineering of Ralstonia eutropha for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose

Paper ID Volume ID Publish Year Pages File Format Full-Text
22942 43401 2015 7 PDF Available
Title
Engineering of Ralstonia eutropha for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose
Abstract

•Seven strains were generated from R. euthropha for PHBV production without propionate addition.•These strains accumulated PHBVs, accounting for 59.4–70.3% of CDW, with 3HV ratios of 0.4–29.5 mol%.•PHBV production and 3HV fraction (in mol%) were 94.4 g/L and 4.8 for Rem-2, and 90.6 g/L and 26.0 for Rem-8, respectively.

Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with Ralstonia eutropha relies on the addition of propionate during fermentation, and propionate consumption is one of the major factors affecting the cost of PHBV production. In this study, 7 strains were obtained by genetic manipulating the methylcitric acid cycle and the methylmalonyl-CoA pathway in R. eutropha. Disruption of prpC1 and prpC2 genes did not affect cell growth and PHBV accumulation. All 7 strains were able to accumulation high amounts of PHBVs with 3HV fractions of 0.41–29.1 mol% during cultivation in flasks. Fermentation in 7.5-L fermenter showed that genetically engineered Rem-8 was able to yield biomass of 132.8 CDW g/L, of which 68.6% were PHBV with 3HV fraction of 26.0 mol% in the biopolymer, indicating promising potentials of commercialization in the future.

Keywords
Ralstonia eutropha; Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV); Propionyl-CoA; 2-Methylcitric acid cycle; Methylmalonyl-CoA
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Engineering of Ralstonia eutropha for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 195, 10 February 2015, Pages 82–88
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us