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Development of a hazel cell culture-based paclitaxel and baccatin III production process on a benchtop scale

Paper ID Volume ID Publish Year Pages File Format Full-Text
22944 43401 2015 10 PDF Available
Title
Development of a hazel cell culture-based paclitaxel and baccatin III production process on a benchtop scale
Abstract

•Process development of taxane production in Corylus avellana cell suspension cultures.•Establishment of taxane production in C. avellana cells in benchtop bioreactors.•An alternative process for taxane production is proposed.•Cor and MeJA highly increase paclitaxel and baccatin III production in C. avellana.•1 μM Cor and pcv = 50% resulted in the maximum taxane concentrations.

The growing demand for the antitumorous agent paclitaxel and the difficulty in increasing its production by genetic engineering has prompted a search for new sources of taxanes. It has been reported that taxanes can be extracted from the angiosperm Corylus avellana L. Our aim was to improve taxane production by scaling up the process from mL-level to benchtop bioreactors, optimizing culture conditions and comparing the effect of two elicitors, 1 μM coronatine (Cor) and 100 μM methyl jasmonate (MeJA).Orbitally shaken flask cultures achieved a maximum fresh cell weight of 11.54 gDCW/L under control conditions, and MeJA- and Cor-treatment produced a statistically significant reduction in growth to 4.28 gDCW/L and 5.69 gDCW/L, while increasing the taxane content 3- and 27-fold, respectively. The enhancing effect of these elicitors on taxane production, despite affecting growth, was confirmed in orbitally shaken TubeSpin® Bioreactors 50, where the highest taxane content (8583.3 μg/L) was obtained when 1 μM Cor was used and elicitation took place at a packed cell volume of 50%. Two benchtop stirred bioreactors, BIOSTAT® B plus and UniVessel® SU, were compared, the latter providing a higher biomass of C. avellana cell suspension cultures. Transferring the established optimum culture conditions for taxane production to the UniVessel® SU resulted in a total taxane content of 6246.1 μg/L, a 10-fold increase compared with shake flask experiments.

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Keywords
C. avellana, Corylus avellana; Cor, coronatine; DCW, dry cell weight; FCW, fresh cell weight; HPLC, high-performance liquid chromatography; MeJA, methyl jasmonate; MeOH, methanol; MS, Murashige and Skoog; pcv, packed cell volume; rcf, relative centrifugal
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Development of a hazel cell culture-based paclitaxel and baccatin III production process on a benchtop scale
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 195, 10 February 2015, Pages 93–102
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us