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Photosynthetic production of glycerol by a recombinant cyanobacterium

Paper ID Volume ID Publish Year Pages File Format Full-Text
22945 43401 2015 6 PDF Available
Title
Photosynthetic production of glycerol by a recombinant cyanobacterium
Abstract

•Recombinant Synechocystis harboring phosphoglycerol phosphatase produces glycerol.•Glycerol is produced at high levels.•Wild-type Synechocystis produces low amounts of glycerol under salt stress.•Glycerol production through the heterologous phosphatase and through salt stress is cumulative.•A sustainable route to glycerol production is provided that does not require arable land.

Cyanobacteria are prokaryotic organisms capable of oxygenic photosynthesis. Glycerol is an important commodity chemical. Introduction of phosphoglycerol phosphatase 2 from Saccharomyces cerevisiae into the model cyanobacterium Synechocystis sp. PCC6803 resulted in a mutant strain that produced a considerable amount of glycerol from light, water and CO2. Mild salt stress (200 mM NaCl) on the cells led to an increase of the extracellular glycerol concentration of more than 20%. Under these conditions the mutant accumulated glycerol to an extracellular concentration of 14.3 mM after 17 days of culturing.

Keywords
Synechocystis; Metabolic engineering; Cell factory; Glycerol
First Page Preview
Photosynthetic production of glycerol by a recombinant cyanobacterium
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 195, 10 February 2015, Pages 46–51
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering