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Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved

Paper ID Volume ID Publish Year Pages File Format Full-Text
22964 43403 2015 11 PDF Available
Title
Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved
Abstract

•We previously demonstrated that epidermal growth factor (EGF) containing supernatant, from the culture of a genetically modified EGF expressing Lactococcus lactis, enhance growth performance of early-weaned pigs. The current study aimed to further understand the physiological and molecular mechanisms behind this improved performance.•The EGF supernatant increased the expression intestine function genes such as glucose cotransporter sodium-glucose linked transporter 1 (SGLT1), and glucagon-like peptide-2 (GLP2), mucin2, goblet cell numbers, and thus intestine development.•The EGF supernatant suppresses early-weaned associate intestine inflammation as reflected by decreased lamina propria width, and higher expression of anti-inflammatory cytokine, IL-13.

We have previously generated epidermal factor expressing Lactococcus lactis (EGF-LL) using a bioengineering approach, and shown that EGF-LL fermentation supernatant enhanced newly weaned pigs growth. The objective of the current study was to further understand the mechanisms behind this improved performance. Sixty-four piglets were weaned at 3 weeks of age and then fed ad libitum according to a 2-phase feeding program. Four pens with 8 pigs per pen were assigned to each of two treatments for 3 weeks: (1) EGF containing supernatant from EGF-LL culture (SuperEGF) or (2) blank M17GE media (Control). Consistent with previous findings, SuperEGF pigs had an increased average daily gain during week 3 post-weaning (433.4 ± 10.86 vs 388.7 ± 7.76 g; P < 0.05) and overall gain:feed ratio (0.757 ± 0.03 vs 0.677 ± 0.01 kg/kg, P < 0.05). Moreover, jejunal structure development was enhanced, and inflammation index was minimized in SuperEGF pigs as indicated by increased villi height (P < 0.05), decreased lamina propria width (P < 0.05), and higher expression of anti-inflammatory cytokine, IL-13 (P < 0.05). Further, goblet cell numbers and Muc2 levels were increased in SuperEGF pigs. Interestingly, the weaning-induced decrease of glucose cotransporter sodium-glucose linked transporter 1 (SGLT1) and glucagon-like peptide-2 (GLP2) levels was reversed by SuperEGF supplementation. Our findings add to our understanding of the mechanisms behind enhancing piglet performance by EGF containing fermentation product.

Keywords
Epidermal growth factor; Weaned pig; Intestine development; Lactococcus lactis
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Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volumes 196–197, 20 February–10 March 2015, Pages 9–19
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us