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Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypii

Paper ID Volume ID Publish Year Pages File Format Full-Text
22967 43408 2015 4 PDF Available
Title
Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypii
Abstract

•Riboflavin overproduction by A. gossypii Agura3 on standard complex medium.•Excess uridine/uracil repressed the production of riboflavin by this auxotroph.•High uracil concentrations were detrimental for the A. gossypii growth.•Excess uridine favored the A. gossypii Agura3 growth.•Alterations in the A. gossypii pyrimidine pathway affected riboflavin production.

The Ashbya gossypii riboflavin biosynthetic pathway and its connection with the purine pathway have been well studied. However, the outcome of genetic alterations in the pyrimidine pathway on riboflavin production by A. gossypii had not yet been assessed. Here, we report that the blockage of the de novo pyrimidine biosynthetic pathway in the recently generated A. gossypii Agura3 uridine/uracil auxotrophic strain led to improved riboflavin production on standard agar-solidified complex medium. When extra uridine/uracil was supplied, the production of riboflavin by this auxotroph was repressed. High concentrations of uracil hampered this (and the parent) strain growth, whereas excess uridine favored the A. gossypii Agura3 growth. Considering that the riboflavin and the pyrimidine pathways share the same precursors and that riboflavin overproduction may be triggered by nutritional stress, we suggest that overproduction of riboflavin by the A. gossypii Agura3 may occur as an outcome of a nutritional stress response and/or of an increased availability in precursors for riboflavin biosynthesis, due to their reduced consumption by the pyrimidine pathway.

Keywords
Ashbya gossypii; Pyrimidine pathway; Riboflavin production; Uridine/uracil auxotrophy; Nutritional stress
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 193, 10 January 2015, Pages 37–40
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us