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Expanding the laccase-toolbox: A laccase from Corynebacterium glutamicum with phenol coupling and cuprous oxidase activity

Paper ID Volume ID Publish Year Pages File Format Full-Text
22997 43409 2014 8 PDF Available
Title
Expanding the laccase-toolbox: A laccase from Corynebacterium glutamicum with phenol coupling and cuprous oxidase activity
Abstract

•New laccase CgL1 from Corynebacterium glutamicum was expressed in E. coli.•CgL1 is stable in the presence of organic solvents and at elevated temperatures.•CgL1 is active at alkaline pH-values.•CC / C-O coupling of phenolic acids as well as oxidation of Cu+ with CgL1 was demonstrated.•Dehydrodimer of sinapic acid is the main product of sinapic acid conversion.

Laccases are oxidases with potential for application in biotechnology. Up to now only fungal laccases have been applied in technical processes, although bacterial laccases are generally easier to handle and more stable at alkaline pH values and elevated temperatures. To increase the toolbox of bacterial laccases and to broaden our knowledge about them, new enzymes have to be characterized. Within this study, we describe the new bacterial laccase CgL1 from Corynebacterium glutamicum. CgL1 was found to oxidize typical laccase substrates like 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), syringaldazine and 2,6-dimethoxyphenol. The enzyme also demonstrates cuprous oxidase activity. Furthermore, CgL1 is active for several hours at temperatures up to 60 °C and at alkaline pH, as well as stable in different organic solvents. This makes CgL1 a potential candidate for technical applications. In addition, CgL1 was found to catalyze the CC/CO coupling of several phenolic compounds which can serve as precursors for the synthesis of natural products like antibiotics and phytohormones. This activity and product distribution were influenced by pH value and mediators used.

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Keywords
Laccase; Corynebacterium glutamicum; Phenol coupling; Dimerization; Cu+ oxidationABTS, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid); SGZ, syringaldazine; 2,6-DMP, 2,6-dimethoxyphenol; MCO, multi-copper oxidase; SDS-PAGE, sodium dodecyl sulfate
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Expanding the laccase-toolbox: A laccase from Corynebacterium glutamicum with phenol coupling and cuprous oxidase activity
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 191, 10 December 2014, Pages 46–53
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us