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Biological significance of nanograined/ultrafine-grained structures: Interaction with fibroblasts

Paper ID Volume ID Publish Year Pages File Format Full-Text
2300 107 2010 10 PDF Available
Title
Biological significance of nanograined/ultrafine-grained structures: Interaction with fibroblasts
Abstract

Given the need to develop high strength/weight ratio bioimplants with enhanced cellular response, we describe here a study focused on the processing–structure–functional property relationship in austenitic stainless steel that was processed using an ingenious phase reversion approach to obtain an nanograined/ultrafine-grained (NG/UFG) structure. The cellular activity between fibroblast and NG/UFG substrate is compared with the coarse-grained (CG) substrate. A comparative investigation of NG/UFG and CG structures illustrated that cell attachment, proliferation, viability, morphology and spread are favorably modulated and significantly different from the conventional CG structure. These observations were further confirmed by expression levels of vinculin and associated actin cytoskeleton. Immunofluorescence studies demonstrated increased vinculin concentrations associated with actin stress fibers in the outer regions of the cells and cellular extensions on NG/UFG substrate. These observations suggest enhanced cell–substrate interaction and activity. The cellular attachment response on NG/UFG substrate is attributed to grain size and hydrophilicity and is related to more open lattice in the positions of high-angle grain boundaries.

Keywords
Nanostructured materials; Phase reversion; Stainless steel; Cellular response; Fibroblasts
First Page Preview
Biological significance of nanograined/ultrafine-grained structures: Interaction with fibroblasts
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 6, Issue 8, August 2010, Pages 3339–3348
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering