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Circadian rhythms in the cell cycle and biomass composition of Neochloris oleoabundans under nitrogen limitation

Paper ID Volume ID Publish Year Pages File Format Full-Text
23027 43413 2014 9 PDF Available
Title
Circadian rhythms in the cell cycle and biomass composition of Neochloris oleoabundans under nitrogen limitation
Abstract

•Also under nitrogen limitation cell division was scheduled by the circadian clock.•Two subpopulations arose in the reactor dividing alternately every other day.•This phased cell division caused changes in biomass yield and composition.•Neutral lipids build up in the day, mostly in cells arrested in their cell cycle.

The circadian clock schedules processes in microalgae cells at suitable times in the day/night cycle. To gain knowledge about these biological time schedules, Neochloris oleoabundans was grown under constant light conditions and nitrogen limitation. Under these constant conditions, the only variable was the circadian clock. The results were compared to previous work done under nitrogen-replete conditions, in order to determine the effect of N-limitation on circadian rhythms in the cell cycle and biomass composition of N. oleoabundans.The circadian clock was not affected by nitrogen-limitation, and cell division was timed in the natural night, despite of constant light conditions. However, because of nitrogen-limitation, not the entire population was able to divide every day. Two subpopulations were observed, which divided alternately every other day. This caused oscillations in biomass yield and composition. Starch and total fatty acids (TFA) were accumulated during the day. Also, fatty acid composition changed during the cell cycle. Neutral lipids were built up during the day, especially in cells that were arrested in their cell cycle (G2 and G3). These findings give insight in the influence of circadian rhythms on the cell cycle and biomass composition.

Keywords
Microalgae; Circadian rhythms; Cell cycle; Biomass composition; Nitrogen limitationBSA, bovine serine albumine; D, dilution rate (day−1); DW, dry weight (g L−1); N0, number of cells per mL of culture at time = 0; Nt, number of cells per mL of culture at t
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Circadian rhythms in the cell cycle and biomass composition of Neochloris oleoabundans under nitrogen limitation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 187, 10 October 2014, Pages 25–33
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us