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Production of a water-soluble fertilizer containing amino acids by solid-state fermentation of soybean meal and evaluation of its efficacy on the rapeseed growth

Paper ID Volume ID Publish Year Pages File Format Full-Text
23028 43413 2014 9 PDF Available
Title
Production of a water-soluble fertilizer containing amino acids by solid-state fermentation of soybean meal and evaluation of its efficacy on the rapeseed growth
Abstract

•The strain Bacillus subtilis N-2 producing protease degraded the protein in soybean meal.•Above 99% of protein was dissolved after solid-state fermentation from soybean meal.•The water-soluble substances containing high content of amino acids can be used as efficient bio-organic fertilizer.

Soybean meal is a by-product of soybean oil extraction and contains approximately 44% protein. We performed solid-state fermentation by using Bacillus subtilis strain N-2 to produce a water-soluble fertilizer containing amino acids. Strain N-2 produced a high yield of protease, which transformed the proteins in soybean meal into peptide and free amino acids that were dissolved in the fermentation products. Based on the Plackett–Burman design, the initial pH of the fermentation substrate, number of days of fermentation, and the ratio of liquid to soybean meal exhibited significant effects on the recovery of proteins in the resulting water-soluble solution. According to the predicted results of the central composite design, the highest recovery of soluble proteins (99.072%) was achieved at the optimum conditions. Under these conditions, the resulting solution contained 50.42% small peptides and 7.9% poly-γ-glutamic acid (γ-PGA). The water-soluble fertilizer robustly increased the activity of the rapeseed root system, chlorophyll content, leaf area, shoot dry weight, root length, and root weight at a concentration of 0.25% (w/v). This methodology offers a value-added use of soybean meal.

Keywords
Soybean meal; Solid-state fermentation; Bacillus subtilis; Protease; Water-soluble fertilizerSSF, solid-state fermentation; WSF, water-soluble fertilizer; TN, total nitrogen; TC, total carbohydrate; RWP, recovery of water-soluble protein
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Production of a water-soluble fertilizer containing amino acids by solid-state fermentation of soybean meal and evaluation of its efficacy on the rapeseed growth
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 187, 10 October 2014, Pages 34–42
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us