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Heterologous production of the lipopeptide biosurfactant serrawettin W1 in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
23053 43414 2014 4 PDF Available
Title
Heterologous production of the lipopeptide biosurfactant serrawettin W1 in Escherichia coli
Abstract

•The non-ionic lipopeptide serrawettin W1 is a biosurfactant produced by Serratia marcescens.•Serrawettin shows antimicrobial, antitumor and plant protecting properties.•We describe a simple method for the heterologous production of serrawettin in Escherichia coli.•Our method paves the way for the large scale production of this biotechnologically interesting biosurfactant.

The non-ionic biosurfactant serrawettin W1 is a lipopeptide produced by red-pigmented strains of Serratia marcescens which shows antimicrobial, antitumor and plant protecting properties. Here, we report a simple method for heterologous production of this biosurfactant. S. marcescens strain DSM12481 was identified as a novel serrawettin W1 producer and the respective nonribosomal peptide synthetase gene swrW was cloned and expressed in Escherichia coli BL21 Gold. Chemical analysis of heterologous serrawettin W1 revealed that E. coli mainly produced serrawettin with C10 fatty acids as does S. marcescens. Additionally, serrawettin species with longer fatty acids (C13, C14) were detected in S. marcescens which were absent in E. coli. The expression system described here paves the way for the large scale production of this biotechnologically important biosurfactant.

Keywords
Serratia marcescens; Serrawettin W1; Biosurfactant; Heterologous expression
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Heterologous production of the lipopeptide biosurfactant serrawettin W1 in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 181, 10 July 2014, Pages 27–30
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us