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Engineered UV-A light-responsive gene expression system for measuring sun cream efficacy in mammalian cell culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
23061 43415 2014 4 PDF Available
Title
Engineered UV-A light-responsive gene expression system for measuring sun cream efficacy in mammalian cell culture
Abstract

•UV-A and blue light-inducible gene expression system.•Mammalian synthetic light-dependent dimerization of CRY2 and C1BN.•Cell-based evaluation of sun cream UV-A protection properties.

Light-dependent gene regulation systems are advantageous as they allow for precise spatio-temporal control of target gene expression. In this paper, we present a novel UV-A and blue-light-inducible gene control system that is based on the light-dependent heterodimerization of the CRY2 and C1BN domains. Upon their interaction, a transcription factor is released from the cell membrane and initiates target gene expression. Capitalizing on that, sun cream UV-A protection properties were measured intracellularly.

Keywords
CIB1, cryptochrome-interacting basic-helix–loop–helix; C1BN, truncated variant of CIB1; FKF1, flavin-binding kelch repeat f-box 1; GI, GIGANTEA; CRY2, cryptochrome 2; TEV, tobacco etch virus proteaseSynthetic biology; Gene control; Protein engineering; Op
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Engineered UV-A light-responsive gene expression system for measuring sun cream efficacy in mammalian cell culture
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 189, 10 November 2014, Pages 150–153
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us