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Formulation of fermentation media from flour-rich waste streams for microbial lipid production by Lipomyces starkeyi

Paper ID Volume ID Publish Year Pages File Format Full-Text
23071 43415 2014 10 PDF Available
Title
Formulation of fermentation media from flour-rich waste streams for microbial lipid production by Lipomyces starkeyi
Abstract

•Confectionary industry waste has been valorised through a novel biorefinery concept.•Nutrient-complete fermentation media were produced from flour-rich waste streams.•Microbial oil production was achieved using crude hydrolysates as the sole fermentation feedstock.•Fed-batch bioreactor cultures led to a microbial oil concentration of 64.5 g/L.

Flour-rich waste (FRW) and by-product streams generated by bakery, confectionery and wheat milling plants could be employed as the sole raw materials for generic fermentation media production, suitable for microbial oil synthesis. Wheat milling by-products were used in solid state fermentations (SSF) of Aspergillus awamori for the production of crude enzymes, mainly glucoamylase and protease. Enzyme-rich SSF solids were subsequently employed for hydrolysis of FRW streams into nutrient-rich fermentation media. Batch hydrolytic experiments using FRW concentrations up to 205 g/L resulted in higher than 90% (w/w) starch to glucose conversion yields and 40% (w/w) total Kjeldahl nitrogen to free amino nitrogen conversion yields. Starch to glucose conversion yields of 98.2, 86.1 and 73.4% (w/w) were achieved when initial FRW concentrations of 235, 300 and 350 g/L were employed in fed-batch hydrolytic experiments, respectively. Crude hydrolysates were used as fermentation media in shake flask cultures with the oleaginous yeast Lipomyces starkeyi DSM 70296 reaching a total dry weight of 30.5 g/L with a microbial oil content of 40.4% (w/w), higher than that achieved in synthetic media. Fed-batch bioreactor cultures led to a total dry weight of 109.8 g/L with a microbial oil content of 57.8% (w/w) and productivity of 0.4 g/L/h.

Keywords
Food waste valorisation; Solid state fermentation; Enzymatic hydrolysis; Lipomyces starkeyi; Microbial oil
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Formulation of fermentation media from flour-rich waste streams for microbial lipid production by Lipomyces starkeyi
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 189, 10 November 2014, Pages 36–45
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us