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In silico identification of potential virulence genes in 1,3-propanediol producer Klebsiella pneumonia

Paper ID Volume ID Publish Year Pages File Format Full-Text
23074 43415 2014 6 PDF Available
Title
In silico identification of potential virulence genes in 1,3-propanediol producer Klebsiella pneumonia
Abstract

•2457 Virulence genes (VFs) were scanned in 9 sequenced K. pneumoniae strains.•We found different numbers and types of VFs in 9 strains.•Four large fragments (VF1-4) with contiguous potential virulence genes were identified.•VF1 and VF2 existed in all 9 sequenced strains and the 1,3-PD producer (KG1).•There was no influence of knockout VF2 in KG1 on cell growth and 1,3-PD production.

The pathogenic characteristics of Klebsiella pneumoniae could pose security risks for industrial applications. In this study, the existence and distribution of 2457 known virulence genes (VFs) in 9 strains of K. pneumoniae were systematically analyzed by high-throughput in silico methods. We found different numbers and types of VFs in 9 K. pneumoniae strains using database sequences. Some VFs in the database were highly homologous with the corresponding genes in K. pneumoniae genomes. Four large fragments with contiguous potential virulence genes named VF1, VF2, VF3 and VF4 were identified. VF1 and VF2 were found in all 9 sequenced strains and the 1,3-propanediol-producing strain KG1. When the VF2 fragment was knocked out in KG1, cell growth and 1,3-propanediol production in the mutant were nearly the same as in KG1. Consequently the resulting information by in silico methods is useful for identifying potential virulence genes of K. pneumoniae used for 1,3-propanediol production.

Keywords
1,3-Propanediol; Potential virulence genes; In silico; Klebsiella pneumonia
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In silico identification of potential virulence genes in 1,3-propanediol producer Klebsiella pneumonia
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 189, 10 November 2014, Pages 9–14
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us