CEM-Designer: Design of custom expression microarrays in the post-ENCODE Era
•We revisit custom expression microarray design in the face of complex transcriptomes.•We provide target selection methods accounting for different transcript biotypes.•We reduce cross-hybridization effects of probes to even yet unannotated targets.•We provide our selection methods as a freely available web server.
Microarrays are widely used in gene expression studies, and custom expression microarrays are popular to monitor expression changes of a customer-defined set of genes. However, the complexity of transcriptomes uncovered recently make custom expression microarray design a non-trivial task. Pervasive transcription and alternative processing of transcripts generate a wealth of interweaved transcripts that requires well-considered probe design strategies and is largely neglected in existing approaches.We developed the web server CEM-Designer that facilitates microarray platform independent design of custom expression microarrays for complex transcriptomes. CEM-Designer covers (i) the collection and generation of a set of unique target sequences from different sources and (ii) the selection of a set of sensitive and specific probes that optimally represents the target sequences. Probe design itself is left to third party software to ensure that probes meet provider-specific constraints.CEM-Designer is available at http://designpipeline.bioinf.uni-leipzig.de.
Journal: Journal of Biotechnology - Volume 189, 10 November 2014, Pages 154–156