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Carbon sink removal: Increased photosynthetic production of lactic acid by Synechocystis sp. PCC6803 in a glycogen storage mutant

Paper ID Volume ID Publish Year Pages File Format Full-Text
23182 43419 2014 3 PDF Available
Title
Carbon sink removal: Increased photosynthetic production of lactic acid by Synechocystis sp. PCC6803 in a glycogen storage mutant
Abstract

•To increase lactate production in engineered Synechocystis we removed endogenous carbon sinks.•No effect on lactate production was observed in a polyhydroxybutyrate synthesis deletion mutant.•Elimination of glycogen synthesis raised production only under nitrogen limiting conditions.•Under optimized conditions, carbon sink removal can be beneficial for production.

Deletion of pathways for carbon-storage in the cyanobacterium Synechocystis sp. PCC6803 has been suggested as a strategy to increase the size of the available pyruvate pool for the production of (heterologous) chemical commodities. Here we show that deletion of the pathway for glycogen synthesis leads to a twofold increased lactate production rate, under nitrogen-limited conditions, whereas impairment of polyhydroxybutyrate synthesis does not.

Keywords
Cyanobacteria; Metabolic engineering; Glycogen; Polyhydroxybutyrate; Lactate
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Carbon sink removal: Increased photosynthetic production of lactic acid by Synechocystis sp. PCC6803 in a glycogen storage mutant
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 184, 20 August 2014, Pages 100–102
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us