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Immobilisation increases yeast cells’ resistance to dehydration–rehydration treatment

Paper ID Volume ID Publish Year Pages File Format Full-Text
23184 43419 2014 3 PDF Available
Title
Immobilisation increases yeast cells’ resistance to dehydration–rehydration treatment
Abstract

•We studied the influence of new immobilisation method which includes a dehydration stage on yeast viability.•We used yeast strains with different resistance to dehydration.•We showed that immobilisation of all strains improved their resistance to dehydration–rehydration treatment.•We suppose that this new method can be used for production and storage of dry active yeast preparations.

This study was performed with the goal of revealing if the dehydration procedure used in our new immobilisation method noticeably decreases the viability of yeast cells in immobilised preparations. Various yeasts were used in this research: Saccharomyces cerevisiae cells that were rather sensitive to dehydration and had been aerobically grown in an ethanol-containing medium, a recombinant strain of S. cerevisiae grown in aerobic conditions which were completely non-resistant to dehydration and an anaerobically grown bakers’ yeast strain S. cerevisiae, as well as a fairly resistant Pichia pastoris strain. Experiments performed showed that immobilisation of all these strains essentially increased their resistance to a dehydration–rehydration treatment. The increase of cells’ viability (compared with control cells dehydrated in similar conditions) was from 30 to 60%. It is concluded that a new immobilisation method, which includes a dehydration stage, does not lead to an essential loss of yeast cell viability. Correspondingly, there is no risk of losing the biotechnological activities of immobilised preparations. The possibility of producing dry, active yeast preparations is shown, for those strains that are very sensitive to dehydration and which can be used in biotechnology in an immobilised form. Finally, the immobilisation approach can be used for the development of efficient methods for the storage of recombinant yeast strains.

Keywords
Dehydration; Immobilisation; Yeast; Viability; Resistance
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 184, 20 August 2014, Pages 169–171
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us