fulltext.study @t Gmail

Characterization and bioassay of post-translationally modified interferon α-2b expressed in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
23190 43419 2014 6 PDF Available
Title
Characterization and bioassay of post-translationally modified interferon α-2b expressed in Escherichia coli
Abstract

•Human interferon α-2b gene in E. coli produces N-terminal variants of the protein.•The nature of the variations was established by mass spectrometry and proteomic.•One of the major species was Nα-acetyl-interferon α-2b.

Examples of N-terminal acetylation are rare in prokaryotic systems, but in this study, we report one such example in which N-terminal Cys residue of recombinant human interferon α-2b produced in Escherichia coli is a favourite site for Nα-acetylation. The recombinant protein following Q-sepharose chromatography gave a single band on PAGE analysis. However, on reverse phase HPLC the material separated into three peaks. These were characterized by mass spectrometric techniques as: (a) the direct translation product of the gene retaining the N-terminal methionine, (b) a species from which the methionyl residue had been removed by E. coli methionyl aminopeptidase to give the native interferon α-2b and (c) in which the N-terminal Cys residue of the latter contained an acetyl group. Tryptic digestion of interferon α-2b gave fragments linking Cys1 to Cys98 and Cys29 to Cys138, while that of Nα-acetyl-interferon α-2b gave the Cys1–Cys98 fragment with an additional mass of 42 attributed to an acetylated N-terminal. Bioassay of the derivatives showed that Nα-acetyl-interferon α-2b had 10% of the activity of interferon α-2b. The results suggest that the lower activity derivative seen here in E. coli may also be produced when the protein is produced in yeast.

Keywords
Mass spectrometry; Nα-acetyl-interferon α-2b; Met-interferon α-2b; N-acetyl transferase; Tryptic mapping
First Page Preview
Characterization and bioassay of post-translationally modified interferon α-2b expressed in Escherichia coli
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 184, 20 August 2014, Pages 11–16
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering