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Cell-induced response by tetracyclines on human bone marrow colonized hydroxyapatite and Bonelike®

Paper ID Volume ID Publish Year Pages File Format Full-Text
2321 108 2008 8 PDF Available
Title
Cell-induced response by tetracyclines on human bone marrow colonized hydroxyapatite and Bonelike®
Abstract

Semi-synthetic tetracyclines are commonly used antibiotics that also seem to play an important role in the modulation of the immuno-inflammatory imbalance, verified in several bone diseases. The association of a therapeutic agent (that prevents bacterial infection and induces tissue formation) to a biomaterial aiming to repair/regenerate bone defects could contribute to a more predictable clinical outcome. The present study intends to evaluate the proliferation and functional activity of osteoblast-induced human bone marrow cells, cultured on the surface of hydroxyapatite (HA) and Bonelike®, in the presence of therapeutic concentrations of doxycycline and minocycline. First passage bone marrow cells were cultured for 35 days on the surface of HA and Bonelike® discs, in the absence or presence of 1 μg ml−1 doxycycline and minocycline. Cultures performed in standard tissue culture plates were used as control.Doxycycline or minocycline induced cell proliferation and increased the extent of matrix mineralization in osteoblastic cell cultures established in the three substrates. Also, an improved biological behavior was verified in seeded Bonelike® compared with HA. The results suggest that the local delivery of tetracyclines might associate the antimicrobial activity in implant-related bone infection with an eventual induction of osteoblastic proliferation and maintenance of the characteristic biological activity of these cells.

Keywords
Tetracyclines; Osteoblasts; Hydroxyapatite; Bonelike
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Cell-induced response by tetracyclines on human bone marrow colonized hydroxyapatite and Bonelike®
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 4, Issue 3, May 2008, Pages 630–637
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us