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A new series of vectors for constitutive, inducible or repressible gene expression in Candida guilliermondii

Paper ID Volume ID Publish Year Pages File Format Full-Text
23213 43420 2014 6 PDF Available
Title
A new series of vectors for constitutive, inducible or repressible gene expression in Candida guilliermondii
Abstract

•The biotechnological potential of C. guilliermondii is now well established.•The number of expression systems in C. guilliermondii remains limited.•We developed a new series of versatile expression vectors for this yeast.•These plasmids will be used to boost biosynthetic routes in C. guilliermondii.

The biotechnological potential of C. guilliermondii is now well established. This yeast species currently benefits from the availability of a convenient molecular toolbox including recipient strains, selectable markers and optimized transformation protocols. However, the number of expression systems for biotechnological applications in C. guilliermondii remains limited. We have therefore developed and characterized a new series of versatile controllable expression vectors for this yeast. While previous studies firmly demonstrated that knock-out systems represent efficient genetic strategies to interrupt yeast biochemical pathways at a specific step in C. guilliermondii, the set of expression plasmids described in this study will provide new powerful opportunities to boost homologous or heterologous biosynthetic routes by fine controlled over-expression approaches.

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Keywords
Constitutive promoter; Inducible promoter; Repressible promoter; Expression vectors; Candida guilliermondii
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A new series of vectors for constitutive, inducible or repressible gene expression in Candida guilliermondii
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 180, 20 June 2014, Pages 37–42
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us